The response of physiological and xylem anatomical traits under cadmium stress in Pinus thunbergii seedlings.

Studying the response of physiological and xylem anatomical traits under cadmium (Cd) stress is helpful to understand plants' response to heavy metal stress. Here, seedlings of Pinus thunbergii were treated with 50, 100 and 150 mg/kg Cd2+ for 28 days. Cd and nonstructural carbohydrate (NSC) content of leaves, stems and roots, root Cd2+ flux, Cd distribution pattern in stem xylem and phloem, stem xylem hydraulic traits, cell wall component fractions of stems and roots, phytohormonal content such as abscisic acid (ABA), gibberellic acid 3 (GA3), molecule -indole-3-acetic acid (IAA) and jasmonic acid (JA) from both leaves and roots, as well as xylem anatomical traits from both stems and roots were measured. Root Cd2+ flux increased from 50 to 100 mmol/L Cd2+ stress, however, decreased at 150 mmol/LCd2+. Cellulose and hemicellulose in leaves, stems and roots did not change significantly under Cd stress, while pectin decreased significantly. The NSC content of both leaves and stems showed significant changes under Cd stress while the root NSC content was not affected. In both leaves and roots, the ABA content significantly increased under Cd stress, while the GA3, IAA and JA-ME content significantly decreased. Both xylem hydraulic conductivity and xylem water potential decreased with Cd stress, however, tracheid diameter and double wall thickness of the stems and roots were not affected. High Cd intensity was found in both the stem xylem and phloem in all Cd stressed treatments. Our study highlighted the in-situ observation of Cd distribution in both the xylem and phloem, and demonstrated the instant response of physiological traits such as xylem water potential, xylem hydraulic conductivity, root Cd2+ flux, NSC content, as well as phytohormonal content under Cd stress, and the less affected traits such as xylem anatomical traits, cellulose and hemicellulose.

Crop detection technologies, mechanical weeding executive parts and working performance of intelligent mechanical weeding: a review.

Weeding is a key link in agricultural production. Intelligent mechanical weeding is recognized as environmentally friendly, and it profoundly alleviates labor intensity compared with manual hand weeding. While intelligent mechanical weeding can be implemented only when a large number of disciplines are intersected and integrated. This article reviewed two important aspects of intelligent mechanical weeding. The first one was detection technology for crops and weeds. The contact sensors, non-contact sensors and machine vision play pivotal roles in supporting crop detection, which are used for guiding the movements of mechanical weeding executive parts. The second one was mechanical weeding executive part, which include hoes, spring teeth, fingers, brushes, swing and rotational executive parts, these parts were created to adapt to different soil conditions and crop agronomy. It is a fact that intelligent mechanical weeding is not widely applied yet, this review also analyzed the related reasons. We found that compared with the biochemical sprayer, intelligent mechanical weeding has two inevitable limitations: The higher technology cost and lower working efficiency. And some conclusions were commented objectively in the end.

Copper oxide nanoparticles alter the uptake and distribution of cadmium through disturbing the ordered structure of the cell wall in Arabidopsis root.

Copper oxide nanoparticles (CuO NPs) influence the uptake of heavy metal ions by plants, but molecular mechanism is still unknown. Here, we proved the mechanism of CuO NPs affecting Cd absorption in Arabidopsis root. 4-d-old seedlings were treated by 10 and 20 mg/L CuO NPs for 3 d, which decreased the contents of cellulose and hemicellulose in roots. Moreover, the contents of some important monosaccharides were altered by CuO NPs, including arabinose, glucose and mannose. Biosynthesis of cellulose and hemicellulose is regulated by cellulose synthase A complexe (CSC) dynamics. The synthesis of tubulin cytoskeleton was inhibited by CuO NPs, which resulted in the decrease of CSCs bidirectional velocities. Furthermore, the arrangement and network of cellulose fibrillar bundles were disrupted by CuO NPs. CuO NPs treatment significantly increased the influx of Cd2+. The accumulation and translocation of Cd were increased by 10 and 20 mg/L CuO NPs treatment. The subcellular distribution of Cd in root cells indicated CuO NPs decrease the enrichment of Cd in cell wall, but increase the enrichment of Cd in soluble fraction and organelle. In light of these findings, we proposed a mechanistic model in which CuO NPs destroy the ordered structure of the cell wall, alter the uptake and distribution of Cd in Arabidopsis.

Persulfidation maintains cytosolic G6PDs activity through changing tetrameric structure and competing cysteine sulfur oxidation under salt stress in Arabidopsis and tomato.

Glucose-6-phosphate dehydrogenases (G6PDs) are essential regulators of cellular redox. Hydrogen sulfide (H2 S) is a small gasotransmitter that improves plant adaptation to stress; however, its role in regulating G6PD oligomerization to resist oxidative stress remains unknown in plants. Persulfidation of cytosolic G6PDs was analyzed by mass spectrometry (MS). The structural change model of AtG6PD6 homooligomer was built by chemical cross-linking coupled with mass spectrometry (CXMS). We isolated AtG6PD6C159A and SlG6PDCC155A transgenic lines to confirm the in vivo function of persulfidated sites with the g6pd5,6 background. Persulfidation occurs at Arabidopsis G6PD6 Cystine (Cys)159 and tomato G6PDC Cys155, leading to alterations of spatial distance between lysine (K)491-K475 from 42.0 Å to 10.3 Å within the G6PD tetramer. The structural alteration occurs in the structural NADP+ binding domain, which governs the stability of G6PD homooligomer. Persulfidation enhances G6PD oligomerization, thereby increasing substrate affinity. Under high salt stress, cytosolic G6PDs activity was inhibited due to oxidative modifications. Persulfidation protects these specific sites and prevents oxidative damage. In summary, H2 S-mediated persulfidation promotes cytosolic G6PD activity by altering homotetrameric structure. The cytosolic G6PD adaptive regulation with two kinds of protein modifications at the atomic and molecular levels is critical for the cellular stress response.

Hydrogen sulfide improves salt tolerance through persulfidation of PMA1 in Arabidopsis.

KEY MESSAGE: A new interaction was found between PMA1 and GRF4. H2S promotes the interaction through persulfidated Cys446 of PMA1. H2S activates PMA1 to maintain K+/Na+ homeostasis through persulfidation under salt stress. Plasma membrane H+-ATPase (PMA) is a transmembrane transporter responsible for pumping protons, and its contribution to salt resistance is indispensable in plants. Hydrogen sulfide (H2S), a small signaling gas molecule, plays the important roles in facilitating adaptation of plants to salt stress. However, how H2S regulates PMA activity remains largely unclear. Here, we show a possible original mechanism for H2S to regulate PMA activity. PMA1, a predominant member in the PMA family of Arabidopsis, has a non-conservative persulfidated cysteine (Cys) residue (Cys446), which is exposed on the surface of PMA1 and located in cation transporter/ATPase domain. A new interaction of PMA1 and GENERAL REGULATORY FACTOR 4 (GRF4, belongs to the 14-3-3 protein family) was found by chemical crosslinking coupled with mass spectrometry (CXMS) in vivo. H2S-mediated persulfidation promoted the binding of PMA1 to GRF4. Further studies showed that H2S enhanced instantaneous H+ efflux and maintained K+/Na+ homeostasis under salt stress. In light of these findings, we suggest that H2S promotes the binding of PMA1 to GRF4 through persulfidation, and then activating PMA, thus improving the salt tolerance of Arabidopsis.

Application of Preoperative Ultrasonography in the Percutaneous Minimally Invasive Repair of Acute Closed Achilles Tendon Rupture.

Percutaneous minimally invasive surgery involving Achilles tendon (AT) repair has the advantages of a low rerupture rate and fewer postoperative complications. However, due to the inability to operate under direct vision, the injury of the small saphenous vein (SSV) and sural nerve (SN) remains largely a high risk involving many challenges. We propose to introduce the preoperative application and advantages of ultrasonography in percutaneous minimally invasive surgery for acute AT rupture. Our results indicated that ultrasonography could locate the position of the SN more accurately and reduce the risk of iatrogenic nerve injury. Compared with the traditional surface markers, the preoperative localization and marking of AT, SSV, and SN in ultrasonography significantly reduced the risk of intraoperative accidental injury to blood vessels and nerves, which could reduce postoperative complications and promote early rehabilitation of patients. We ultimately exploit the properties of ultrasonography in percutaneous minimally invasive surgery to treat Achilles tendon rupture.

Nitric oxide induces S-nitrosylation of CESA1 and CESA9 and increases cellulose content in Arabidopsis hypocotyls.

Nitric oxide (NO), a small signaling gas molecule, participates in several growth and developmental processes in plants. However, how NO regulates cell wall biosynthesis remains unclear. Here, we demonstrate a positive effect of NO on cellulose content that may be related to S-nitrosylation of cellulose synthase 1 (CESA1) and CESA9. Two S-nitrosylated cysteine (Cys) residues, Cys562 and Cys641, which are exposed on the surface of CESA1 and CESA9 and located in the cellulose synthase catalytic domain, were identified to be S-nitrosylated. Meanwhile, Cys641 was located on the binding surface of CESA1 and CESA9, and Cys562 was very close to the binding surface. Cellulose synthase complexes (CSCs) dynamics are closely associated with cellulose content. S-nitrosylation of CESA1 and CESA9 improved particles mobility and thus increased the accumulation of cellulose in Arabidopsis hypocotyl cells. An increase in hemicellulose content as well as an alteration in pectin content facilitated cell wall extension and contributed to cell growth, finally promoting elongation of Arabidopsis hypocotyls. Overall, our work provides a path to investigate the way NO affects the cellulose content of plants.

Effect of organic acids and soil particle size on heavy metal removal from bulk soil with washing.

To evaluate the effect of soil particle size on heavy metals removal by washing, two soil samples were collected around a lead-zinc mining area (SM) and lead-zinc smelter (SS). The total content of Cd, Pb and Zn in SM and SS were determined. And the effect of soil particle size on Cd removal by low molecular organic acids was studied. The results showed that Cd was the main pollutant and the total content of Cd in SS can reach to 24.8 mg Kg-1. 68.4% of the total Cd in SM existed in the form of residual state, while 54.7% of the total Cd in SS was in weak acid extractable state. About 50.0% of the Cd distributed in < 2 µm soil size fraction. The washing results indicated that citric acid was a highly efficient eluent among the five low molecular weight organic acids (citric acid, malic acid, tartaric acid, oxalic acid and acetic acid). After washing, 40% and 69.6% of the total Cd in SS and SM can be removed by citric acid, respectively. While only 18.7-40.2% and 32.6-68.7% of Cd was removed from different size fractions of SM and SS, respectively. The species of Cd in soil size fractions affected the removal effect of citric acid. The citric acid can easily remove the weak acid extractable and reducible form of Cd in soil. After eluted by citric acid, the bioavailability of Cd in soil decreased markedly, and the highest decreasing rate reached 93%.

Kinesiophobia could affect shoulder function after repair of rotator cuff tears.

PURPOSE: Kinesiophobia (fear of movement) is a major limiting factor in the return to pre-injury sport level after surgery of rotator cuff tears. The study aims to gain insights into how kinesiophobia affects shoulder pain and function after the repair of full-thickness rotator cuff tears. METHODS: A prospective study was conducted to evaluate patients who underwent rotator cuff repair between January 2019 and December 2019 in our institution. The patients were divided into a trial group with a high kinesiophobia (Tampa Scale for Kinesiophobia [TSK], TSK > 37) and a control group with a low kinesiophobia (TSK ≤ 37). The indicators of interest included the Constant-Murley scores, numerical rating scale (NRS), visual analogue scale (VAS), Oxford Shoulder Score (OSS), and the American shoulder and elbow score (ASES), shoulder function and strength, and range of motion (ROM) at 3 days, 6 weeks, and 12 months after repair of full-thickness rotator cuff tears. RESULTS: In total, 49 patients who underwent repair of full-thickness rotator cuff tears were enrolled, which was divided into a trial group involving 26 patients (mean TSK 52.54) and a control group involving 23 patients (mean TSK 33.43). There were no statistically significant differences in basic information such as age, gender, and length of stay in the two groups. The preoperative and early postoperative functional scores and the Tampa Scale for Kinesiophobia were statistically significant differences between the two groups. However, long-term postoperative follow-up showed no statistically significant difference in ASES, and Constant-Murley scores, OSS, and VAS scores between the two groups as the kinesiophobia changed from positive to negative. CONCLUSION: Degree of kinesiophobia reduced during post-operative rehabilitation of rotator cuff repair patients, but high kinesiophobia is still present in a large portion of the patients after rotator cuff repair. Patients after rotator cuff repair will benefit from early recognition and prevention of kinesiophobia.

Marked changes in biochar's ability to directly immobilize Cd in soil with aging: implication for biochar remediation of Cd-contaminated soil.

To investigate the change in biochar's ability to directly immobilize Cd in soil, a successive wheat cultivation experiment was conducted. Three biochars with different Cd adsorption mechanisms were added to the soils, and a mesh bag was used to separate the soil particles (> 1 µm) from the biochar. The results showed that the ash contents and anionic contents (CO32- and PO43-) of the biochar decreased with the cultivation time, while the oxygen-containing functional group content and CEC of the biochar increased. As a result, the Cd concentration on biochar decreased, by 68.9% for WBC300, while unstable Cd species (acid soluble and reducible fraction of Cd) on biochar increased with successive cultivation, increasing from 3 to 17% for WBC300 in FS. Correspondingly, the ability of biochar to inhibit Cd accumulation in wheat decreased. The results of this study illustrated that the ability of biochar to directly immobilize Cd in soil is not permanent; it gradually decreases with aging in soil. The adsorption mechanism of Cd on biochar changed from precipitation to complexation, and ion exchange processes could be the main reason.

Effects of urease-producing bacteria and eggshell on physiological characteristics and Cd accumulation of pakchoi (Brassica chinensis L.) plants.

Soil cadmium (Cd) contamination resulting from anthropogenic activity poses severe threats to food safety and human health. In this study, a pot experiment was performed to evaluate the possibility of using urease-producing bacterium UR21 and eggshell (ES) waste for improving the physiological characteristics and reducing Cd accumulation of pakchoi (Brassica chinensis L.) plants. UR21 has siderophore and IAA production ability. The application of UR21 and ES individually or in combination could improve the root and shoot length, and fresh and dry weight of pakchoi plants under Cd stress. In Cd + ES + UR21-treated plants, the dry weight of shoot and root were increased by 61.54% and 72.73%, respectively. The chlorophyll a, chlorophyll b, and carotenoid content were increased by 52.19%, 42.95%, and 95.56% in Cd + ES + UR21-treated plants. Meanwhile, the H2O2 and MDA content were decreased while the SOD and POD activity were increased, and an increase of soluble protein level in pakchoi plants was observed under Cd + ES + UR21 treatment. Importantly, eggshell and UR21 alone or in combination induced a decline of Cd content in pakchoi plants, especially that Cd + ES + UR21 treatment decreased Cd content in shoot and root by 26.96% and 42.91%, respectively. Meanwhile, the soil urease and sucrase activities were enhanced. Generally, the combined application of ureolytic bacteria UR21 and eggshell exhibited better effects than applied them individually in terms of alleviating Cd toxicity in pakchoi plants. Our findings may give a unique perspective for an eco-friendly and sustainable strategy to remediate heavy metal-polluted soils.

The potential effectiveness of mixed bacteria-loaded biochar/activated carbon to remediate Cd, Pb co-contaminated soil and improve the performance of pakchoi plants.

Cadmium (Cd) and lead (Pb) are toxic heavy metals that cause severe soil pollution and pose health risks to humans. It is urgent to develop feasible strategies for Pb and Cd remediation. In this study, a bacteria consortium (Enterobacter asburiae G3, Enterobacter tabaci I12 and Klebsiella variicola J2 in a 1:3:3 proportion) with optimal Cd, Pb adsorption ability was constructed and immobilized on biochar (BC)/activated carbon (AC) via physisorption and sodium alginate encapsulation. The effects of mixed bacteria-loaded BC/AC on Cd and Pb remediation were investigated. The results indicated that their application reduced the DTPA-extractable Cd, Pb in soil by 22.05%-55.84% and 31.64%-48.13%, respectively. The residual Pb, Cd were increased while the exchangeable fractions were decreased. Soil urease, catalase and phosphatase activities were enhanced and soil bacterial community was improved, indicating a soil quality improvement. Consequently, the biomass of pakchoi plants was significantly increased. Cd and Pb in the shoots of pakchoi plants were decreased by 28.68%-51.01% and 24.18%-52.87%, respectively. Collectively, the bacteria-loaded BC/AC showed superior performance than free bacteria, BC and AC alone. Our study may provide a better understanding of the development of green and sustainable materials for remediation of heavy metal by the combination of BC/AC and functional bacteria.

A modified single-endobutton technique combined with nice knot for treatment of Rockwood type III or V acromioclavicular joint dislocation.

PURPOSE: Double-endobutton technique, as a widely accepted strategy for the treatment of acromioclavicular joint dislocation, is undergoing constant improvement. This study aims to assess the clinical effect of a modified single-endobutton combined with the nice knot in the fixation of Rockwood type III or V acromioclavicular joint dislocation. METHODS: From January 2016 to June 2019, 16 adult patients (13 males and 3 females) with Rockwood type III or V acromioclavicular joint dislocation were treated with a modified single-endobutton technique combined with the nice knot in our department. The age ranged from 18 to 64 years old with an average of 32.8 years old. Operative time, intraoperative blood loss, post-operative clinical outcomes and radiographic results were recorded and analyzed. Preoperative and last follow-up scores in the Constant-Murley Scale, Neer score, Rating Scale of the American Shoulder and Elbow Surgeons and VAS scale and complications such as infection, re-dislocation, implant loosening, medical origin fracture and hardware pain were recorded and evaluated. RESULTS: Sixteen patients were followed up for 6 to 18 months with an average of 10.3 months. The operative time was 50-90 min with an average of (62.5 ± 3.10) min. The intraoperative blood loss was 30-100 ml, with an average of (55.0 ± 4.28) ml. The complications, such as wound infection, internal fixation failure and fractures, were not found in these cases. According to Karlsson criteria, there were excellent in 14 cases, good in 2 cases at the final follow-up. The mean VAS score of the patients was 5.88 ± 0.26 preoperatively, compared with 0.19 ± 0.14 at the final follow-up evaluation. The difference was statistically significant (P < 0.05). The mean Constant score was 45.5 ± 2.0 preoperatively, compared to 94.0 ± 0.73 at the final follow-up evaluation. The difference was statistically significant (P < 0.05). Patients had statistically significant preoperative and postoperative AC (acromioclavicular distance) and CC (coracoclavicular distance) distances (P < 0.05); 6 months postoperatively the AC(P = 0.412) and CC(P = 0.324) distances were not statistically significant compared to the healthy side. CONCLUSION: Nice knot provides a reliable fixation for the single-endobutton technique in the treatment of acromioclavicular dislocations. The modified single-endobutton technique combined with the nice knot can achieve good clinical outcomes in the treatment of Rockwood type III or V acromioclavicular joint dislocation.

Incorporating in vitro bioaccessibility into human health risk assessment of heavy metals and metalloid (As) in soil and pak choi (Brassica chinensis L.) from greenhouse vegetable production fields in a megacity in Northwest China.

The rapid development of greenhouse vegetable production (GVP) in densely populated areas may cause the heavy metal/metalloid accumulation in soil and pose a threat to human health. In this study, 180 pairs of topsoil and pak choi (Brassica chinensis L.) samples were collected from GVP fields in Xi'an city in Northwest China to analyze health risks of Cd, Cr, Pb, and As in soil and pak choi combining in vitro bioaccessibility investigation. The results showed that Cd and Cr were common pollutants in both soil and pak choi. In the soil-pak choi system, the indexes of non-carcinogenic and carcinogenic risk for adults and children were 1.53, 2.68, and 1.37 × 10-4, 8.14 × 10-5, respectively, thereby indicating the presence of heavy metal/metalloid health risks for both groups. Based on the results, procedures to mitigate heavy metal/metalloid contamination risks should be discussed more during the development of GVP in the largest city in Northwest China.

The Treatment of Subtrochanteric Fracture with Reversed Contralateral Distal Femoral Locking Compression Plate (DF-LCP) Using a Progressive and Intermittent Drilling Procedure in Three Osteopetrosis Patients.

OBJECTIVE: To describe the application of reversed contralateral distal femoral locking compression plate (DF-LCP) inserted through a progressive and intermittent drilling procedure in the treatment of osteopetrotic subtrochanteric fracture (OSF). METHODS: Three patients (one male and two females with an average age of 45.33 ± 11.09 years) with OSF hospitalized between September 2015 and September 2020, were included in this present study. Lateral approach was applied in all patients who accepted open reduction and internal fixation (ORIF) with a reversed contralateral DF-LCP inserted through a progressive and intermittent drilling procedure. The operation time and intraoperative blood loss were recorded to evaluate the efficiency of this surgical method. Physical examination and imaging examination of the fracture site were used to evaluate the fracture union status, the position and stability of the implant, and the alignment of the injured limb at 1, 3, 6, and 12 months after operation, then a subsequent visit was conducted at least once a year. Harris Hip Score (HHS) was used to evaluate the hip joint function at 6 and 12 months after operation. RESULTS: The average operation time was 140 ± 21.60 min (110, 160, and 150 min); The average intraoperative blood loss was about 333.33 ± 23.57 ml (300, 350, and 350 ml). The average follow-up time was 22.33 ± 7.41 months (29, 26, and 12 months). All patients achieved bone union with an average time of 6.67 ± 0.94 months (6, 8, and 6 months). At the time of 6 months after operation, case 1 and 3 were almost pain-free and could walk with full weight bearing while case 2 could walk only with partial weight bearing using a crutch. The HHS scores of cases 1, 2, and 3 were 84/100, 74/100, and 92/100, respectively. At the follow-up at 12 months after operation, the HHS score improved to 91/100, 81/100, and 96/100, respectively. The contralateral incomplete old subtrochanteric fracture was deteriorated in case 1 at 26 months after operation. After 3 months of limited weight bearing using a crutch, bone union was verified in radiograph imaging. Fresh contralateral subtrochanteric fracture occurred in case 2 at 26 months after operation, which was treated using a similar surgical approach, and its clinical outcome is under follow-up. Moreover, no perioperative complications including operation-related death, vascular/nerve injury, deep venous thrombosis, pulmonary embolism, and incision infection, and long-term complications involving malunion, nonunion, implant failure, ankylosis, heterotopic ossification, osteonecrosis, and osteomyelitis were identified. CONCLUSION: The application of reversed contralateral DF-LCP in OSF is practicable and reliable. Progressive and intermittent drilling is a safe and efficient method for implant insertion in this complicated situation.

Persulfidation-induced structural change in SnRK2.6 establishes intramolecular interaction between phosphorylation and persulfidation.

Post-translational modifications (PTMs), including phosphorylation and persulfidation, regulate the activity of SNF1-RELATED PROTEIN KINASE2.6 (SnRK2.6). Here, we report how persulfidations and phosphorylations of SnRK2.6 influence each other. The persulfidation of cysteine C131/C137 alters SnRK2.6 structure and brings the serine S175 residue closer to the aspartic acid D140 that acts as ATP-γ-phosphate proton acceptor, thereby improving the transfer efficiency of phosphate groups to S175 to enhance the phosphorylation level of S175. Interestingly, we predicted that S267 and C137 were predicted to lie in close proximity on the protein surface and found that the phosphorylation status of S267 positively regulates the persulfidation level at C137. Analyses of the responses of dephosphorylated and depersulfidated mutants to abscisic acid and the H2S-donor NaHS during stomatal closure, water loss, gas exchange, Ca2+ influx, and drought stress revealed that S175/S267-associated phosphorylation and C131/137-associated persulfidation are essential for SnRK2.6 function in vivo. In light of these findings, we propose a mechanistic model in which certain phosphorylations facilitate persulfidation, thereby changing the structure of SnRK2.6 and increasing its activity.

Intraoperative Nice knots assistance for reduction in displaced comminuted clavicle fractures.

PURPOSE: The Nice knots have been widely used in orthopedic surgeries to fix torn soft tissue and fracture in recent years. The study aims to investigate the clinical efficacy and prognosis of intraoperative and postoperative Nice Knots-assisted reduction in the treatment of displaced comminuted clavicle fracture. METHODS: From Jan 2014 to Dec 2019, 75 patients diagnosed with unilateral closed displaced comminuted clavicle fracture were treated with open reduction and internal fixation (ORIF) in this study. Nice knot group (the NK group) included 38 patients and the other 37 patients were in the traditional group (the TK group). The time of operation and the amount of bleeding during operation were recorded. Post-operative clinical outcomes and radiographic results were recorded and compared between these two groups. The Visual Analogue Scale (VAS), Neer score, Rating Scale of the American Shoulder and Elbow Surgeons, Constant-Murley score and complications such as infection, nonunion, implant loosening, fragment displacement and hardware pain were observed in the two groups. RESULTS: In the comparison between the two groups, there was no significant difference in age, sex, the cause of displaced clavicle fracture, and other basic information between the two groups. The operation time, intraoperative fluoroscopy time, and intraoperative blood loss were significantly reduced in the NK group (P < 0.01). There were 2 cases of plate fracture in the TK group. The follow-up results showed that there was no significant difference in VAS, Neer score, ASES, and Constant-Murley scores between the two groups. CONCLUSION: The use of Nice knot, in comminuted and displaced clavicle fractures can reduce intraoperative blood loss, shorten operation time, facilitate intraoperative reduction, and achieve satisfactory postoperative clinical results. This study demonstrates that Nice knot is a simple, safe, practical and effective auxiliary reduction method.

Study of soil microorganisms modified wheat straw and biochar for reducing cadmium leaching potential and bioavailability.

The application of crops straw and biochar in trace metals remediation from the contaminated environment attracted more and more attention during the past decade. Although there has been some review work on the mechanism of trace metals stabilization by crops straw, the effects and mechanisms of interaction among soil indigenous-microbes and crops-straw for trace metal adsorption and stabilization is still unclear. In this study, the dynamic effects along with potential mechanisms of wheat-straw (WS), wheat-straw biochar (WBC) and biologically modified wheat-straw (BMWS) were conducted to investigate the adsorption, leaching behaviour, chemical fractions and bioavailability of cadmium (Cd). The results showed that the biosorption capacity (qe) was most elevated in the BMWS treatment (14.42 mg g-1) as compared to WBC (6.28 mg g-1) and WS (4.20 mg g-1). The application of BMWS, WBC and WS at the rate of 3% significantly reduced Cd concentration in leachate to 53, 45 and 21% respectively, as compared to control. The addition of BMWS reduced the exchangeable Cd fraction resulted an increase in organic matter and carbonate bound Cd fraction in the soil. The DTPA extractable Cd was significantly decreased by 31.2 and 28.6% with the application of BMWS and WBC at 3% w/w respectively as compared to control. The research results may provide a novel perceptive for the development of functional materials and strategies for eco-friendly and sustainable trace metal remediation in contaminated soil and water by combination of straw and soil-indigenous microorganisms.

Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.

Cadmium (Cd) is harmful to plant growth and can be easily transferred from soil to plants. Plant cell wall plays important role in preventing Cd from entering cells. Salicylic acid (SA) mediated defense response increases plant resistance to heavy metals. In this study, all tomato seedlings were pre-treated with 100 µM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress. The results showed that exogenous SA significantly reduced Cd accumulation in tomato plants and changed Cd distribution. By analyzing the cell wall composition, it was found cellulose, hemicellulose, pectin, and lignin were induced by SA. Interestingly, the content of Cd in pectin decreased by SA pretreatment, however it was increased in cellulose. Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes. In addition, SA down-regulated the activity of pectin methylesterase. These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through. Furthermore, SA decreased pectin methylesterase activity and content to reduce cell wall Cd accumulation and change the Cd partition ratio.

Rhizosphere iron and manganese-oxidizing bacteria stimulate root iron plaque formation and regulate Cd uptake of rice plants (Oryza sativa L.).

Iron plaque is the amorphous and/or crystalline layer of Fe and Mn (hydr)oxides formed on the root surface of wetland plants. It could adsorb and co-precipitate metal(loid)s at the rhizosphere, thus modulating the uptake and accumulation of metal elements in plants. In this study, the Fe(II)/Mn(II)-oxidizing bacteria Burkholderia sp. D416 (D416) and Pseudomonas sp. YGL (YGL) were isolated from Cd-contaminated rice field, both hydroponic experiment and pot experiment were performed to assess the impact of bacterial inoculation on iron plaque formation, elemental content of the plaque, plant dry mass, antioxidant enzyme activity and Cd content in rice plants. The results revealed that inoculation with D416, YGL, and D416+YGL stimulated iron plaque formation on the root surface of the hydroponic rice. The content of C, N, O, Na, Mg, Al, Si, P, S, Cl, K, Fe and Ca in the root plaque were affected by the bacterial inoculation and varied among different plant growth stages. The pot experiment indicated that inoculation with D416 increased the root dry biomass by 58.89%, and the combined inoculation of D416 and YGL increased the dry biomass of root, shoot and grain by 16.89%, 21.66% and 23.26%, respectively. Importantly, YGL inoculation decreased the Cd translocation from root to shoot and from glume to brown rice grain by 50.00% and 50.27%, respectively, and the Cd content in shoot and brown rice grain were decreased by 20.00% and 34.48%, respectively. Taken together, the elemental content of the iron plaque and Cd content in rice plants varied among different plant growth stages and when plants were inoculated with different bacterial strains. YGL dramatically reduced the Cd content in brown rice grain, thus it could potentially be used to reduce Cd content in rice crop grown in Cd-contaminated soils.